Zinc induces DNA damage in tobacco roots

0399938 - ÚEB 2014 RIV NL eng J - Journal Article
Procházková, Dagmar - Wilhelmová, Naděžda - Pavlíková, D. - Száková, J. - Gichner, Tomáš
Zinc induces DNA damage in tobacco roots.
Biologia Plantarum. Roč. 57, č. 4 (2013), s. 783-787. ISSN 0006-3134. E-ISSN 1573-8264
R&D Projects: GA ČR(CZ) GAP501/11/1239
Institutional research plan: CEZ:AV0Z50380511
Keywords : comet assay * ethyl methanesulphonate * Nicotiana tabacum
Subject RIV: EF - Botanics
Impact factor: 1.740, year: 2013

We applied the alkaline version of the single-cell gel electrophoresis (comet assay) to seedlings of heterozygous tobacco (Nicotiana tabacum L. var. xanthi) treated with zinc acetate dihydrate (20 to 80 mM Zn2+ for 2 h or 2 to 12 mM Zn2+ for 24 h). A dose dependent increase in DNA damage expressed by the tail moment values were observed in nuclei isolated from the roots after 2 and 24 h Zn2+ treatments. In contrast, Zn2+ did not induce significant DNA damage to leaf nuclei, with the exception of 10 or 12 mM Zn2+ for 24 h. Somatic mutations, identified as dark green, yellow, and dark green/yellow double sectors on the pale green tobacco leaves were not detected after any Zn2+ treatments. The accumulation of Zn in roots and shoots was determined by inductively coupled plasma optical emission spectrometry and the Zn content in roots was about three times higher than in shoots.
Permanent Link: http://hdl.handle.net/11104/0227105