Spontaneous mutation 7B-1 in tomato impairs blue light-induced stomatal opening

0399105 - ÚEB 2014 RIV IE eng J - Journal Article
Hlavinka, J. - Nauš, J. - Fellner, Martin
Spontaneous mutation 7B-1 in tomato impairs blue light-induced stomatal opening.
Plant Science. Roč. 209, č. 2013 (2013), s. 75-80. ISSN 0168-9452. E-ISSN 1873-2259
R&D Projects: GA ČR GD522/08/H003
Grant - others:GA ČR(CZ) GAP501/10/0785
Program: GA
Institutional research plan: CEZ:AV0Z50380511
Keywords : ABA * Anion-channel blocker * Blue light
Subject RIV: ED - Physiology
Impact factor: 4.114, year: 2013

It was reported earlier that 7B-1 mutant in tomato (Solanum lycopersicum L.), an ABA overproducer, is defective in blue light (BL) signaling leading to BL-specific resistance to abiotic and biotic stresses. In this work, we examine responses of stomata to blue, red and white lights, fusicoccin, anion channel blockers (anthracene-9-carboxylic acid; 9-AC and niflumic acid; NIF) and ABA. Our results showed that the aperture of 7B-1 stomata does not increase in BL, suggesting that 7B-1 mutation impairs an element of BL signaling pathway involved in stomata]. opening. Similar stomatal responses of 7B-1 and wild type (WT) to fusicoccin or 9-AC points out that activity of H+-ATPase and 9-AC-sensitive anion channels per se is not likely affected by the mutation. Since 9-AC restored stomatal opening of 7B-1 in BL, it seems that 9-AC and BL could block similar type of anion channels. The stomata of both genotypes did not respond to NIF neither in darkness nor in any light conditions tested. In light, 9-AC but not NIF restored stomatal opening inhibited by ABA in WT and 7B-1. We suggest that in comparison to WT, the activity of S-type anion channels in 7B-1 is more promoted by increased ABA content, and less reduced by BL, because of the mutant resistance to BL.
Permanent Link: http://hdl.handle.net/11104/0226486