The role of cytokinins during micropropagation of wych elm

0396876 - ÚEB 2014 RIV NL eng J - Journal Article
Malá, J. - Máchová, P. - Cvrčková, H. - Karady, Michal - Novák, Ondřej - Mikulík, Jaromír - Dostál, J. - Strnad, Miroslav - Doležal, Karel
The role of cytokinins during micropropagation of wych elm.
Biologia Plantarum. Roč. 57, č. 1 (2013), s. 174-178. ISSN 0006-3134. E-ISSN 1573-8264
R&D Projects: GA MZe QI92A247
Grant - others:GA MŠk(CZ) ED0007/01/01
Program: ED
Institutional research plan: CEZ:AV0Z50380511
Keywords : benzylaminopurine * chlorophyll fluorescence * meta-topolin
Subject RIV: GK - Forestry
Impact factor: 1.740, year: 2013

We have compared the influence of two aromatic cytokinin derivatives, N (6)-benzyladenine (BA) and meta-topolin (mT), on the in vitro multiplication and senescence of wych elm (Ulmus glabra Huds.). After 3 months of cultivation, the micropropagation rate was higher (approx. six times more shoots developed) on Murashige and Skoog (MS) medium supplemented with mT than on MS supplemented with BA. Quantification of 50 endogenous cytokinins, using a recently developed UPLC-ESI(+)-MS/MS method, showed significant differences in the cytokinin metabolites (especially different glucosides) in explants cultivated on media supplemented with BA or mT. Differences in efficiency of photosystem II was also detected.
Permanent Link: http://hdl.handle.net/11104/0224562