ML3: a novel regulator of herbivory-induced responses in Arabidopsis thaliana

0395463 - ÚEB 2014 RIV GB eng J - Journal Article
Fridborg, I. - Johansson, A. - Lagensjo, J. - Leelarasamee, N. - Floková, Kristýna - Tarkowská, Danuše - Meijer, J. - Bejai, S.
ML3: a novel regulator of herbivory-induced responses in Arabidopsis thaliana.
Journal of Experimental Botany. Roč. 64, č. 4 (2013), s. 935-948. ISSN 0022-0957. E-ISSN 1460-2431
R&D Projects: GA AV ČR KAN200380801
Grant - others:GA MŠk(CZ) ED0007/01/01
Program: ED
Institutional research plan: CEZ:AV0Z50380511
Keywords : Arabidopsis thaliana * herbivory * jasmonic acid
Subject RIV: EC - Immunology
Impact factor: 5.794, year: 2013

ML (MD2-related lipid recognition) proteins are known to enhance innate immune responses in mammals. This study reports the analysis of the putative ML gene family in Arabidopsis thaliana and suggests a role for the ML3 gene in herbivory-associated responses in plants. Feeding by larvae of the Lepidopteran generalist herbivore Spodoptera littoralis and larvae of the specialist herbivore Plutella xylostella activated ML3 transcription in leaf tissues. ML3 loss-of-function Arabidopsis plants were compromised in the upregulation of herbivory-induced genes and displayed a semi-dwarf phenotype. Herbivory bioassays showed that larvae of S. littoralis fed on ml3 mutant plants gained more weight compared to larvae fed on wild-type plants while larvae of P. xylostella did not show any significant difference. Virus-induced gene silencing of ML3 expression in plants compromised in jasmonic acid (JA) and salicylic acid (SA) signalling revealed a complex role of ML3 in JA/defence signalling affecting both JA- and SA-dependent responses. The data suggest that ML3 is involved in herbivory-mediated responses in Arabidopsis and that it has a potential role in herbivory-associated molecular pattern recognition.
Permanent Link: http://hdl.handle.net/11104/0223485