Heat Shock-Induced Accumulation of Translation Elongation and Termination Factors Precedes Assembly of Stress Granules in S. cerevisiae

0392739 - MBÚ 2014 RIV US eng J - Journal Article
Groušl, Tomáš - Malcová, Ivana - Pompach, Petr - Frýdlová, Ivana - Slabá, Renata - Senohrábková, Lenka - Nováková, Lenka - Hašek, Jiří
Heat Shock-Induced Accumulation of Translation Elongation and Termination Factors Precedes Assembly of Stress Granules in S. cerevisiae.
PLoS ONE. Roč. 8, č. 2 (2013), e57083. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR GA204/09/1924; GA ČR GAP305/12/0480; GA ČR GPP305/10/P253
Institutional support: RVO:61388971
Keywords : CYTOPLASMIC PROCESSING BODIES * GUANINE-NUCLEOTIDE EXCHANGE * EUKARYOTIC MESSENGER-RNAS
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.534, year: 2013

In response to severe environmental stresses eukaryotic cells shut down translation and accumulate components of the translational machinery in stress granules (SGs). Since they contain mainly mRNA, translation initiation factors and 40S ribosomal subunits, they have been referred to as dominant accumulations of stalled translation preinitiation complexes. Here we present evidence that the robust heat shock-induced SGs of S. cerevisiae also contain translation elongation factors eEF3 (Yef3p) and eEF1B gamma 2 (Tef4p) as well as translation termination factors eRF1 (Sup45p) and eRF3 (Sup35p). Despite the presence of the yeast prion protein Sup35 in heat shock-induced SGs, we found out that its prion-like domain is not involved in the SGs assembly
Permanent Link: http://hdl.handle.net/11104/0221528