Nanoparticle-specific changes in Arabidopsis thaliana gene expression after exposure to ZnO, TiO2, and fullerene soot

0389142 - ÚEB 2013 RIV NL eng J - Journal Article
Landa, Přemysl - Vaňková, Radomíra - Andrlová, Jana - Hodek, J. - Maršík, Petr - Štorchová, Helena - White, J. C. - Vaněk, Tomáš
Nanoparticle-specific changes in Arabidopsis thaliana gene expression after exposure to ZnO, TiO2, and fullerene soot.
Journal of Hazardous Materials. Roč. 241, Feb 2012 (2012), s. 55-62. ISSN 0304-3894. E-ISSN 1873-3336
R&D Projects: GA MŠMT(CZ) LH11047
Institutional research plan: CEZ:AV0Z50380511
Keywords : Microarray * Nanoparticles * Gene expression
Subject RIV: DN - Health Impact of the Environment Quality
Impact factor: 3.925, year: 2012

The effect of exposure to 100 mg/L zinc oxide (nZnO), fullerene soot (FS) or titanium dioxide (nTiO(2)) nanoparticles on gene expression in Arabidopsis thaliana roots was studied using microarrays. After 7d, nZnO, FS, or nTiO(2) exposure resulted in 660 up- and 826 down-regulated genes, 232 up- and 189 down-regulated genes, and 80 up- and 74 down-regulated genes, respectively (expression difference > 2-fold: p[t test] < 0.05). The genes induced by nZnO and FS include mainly ontology groups annotated as stress responsive, including both abiotic (oxidative, salt, water deprivation) and biotic (wounding and defense to pathogens) stimuli. The down-regulated genes upon nZnO exposure were involved in cell organization and biogenesis, including translation, nucleosome assembly and microtubule based process. FS largely repressed the transcription of genes involved in electron transport and energy pathways. Only mild changes in gene expression were observed upon nTiO(2) exposure, which resulted in up- and down-regulation of genes involved mainly in responses to biotic and abiotic stimuli. The data clearly indicate that the mechanisms of phytotoxicity are highly nanoparticle dependent despite of a limited overlap in gene expression response.
Permanent Link: http://hdl.handle.net/11104/0218083