Magnetic particles–based biosensor for biogenic amines using an optical oxygen sensor as a transducer

0388384 - ÚVGZ 2013 RIV AT eng J - Journal Article
Pospišková, K. - Šafařík, Ivo - Šebela, M. - Kuncová, Gabriela
Magnetic particles–based biosensor for biogenic amines using an optical oxygen sensor as a transducer.
Microchimica Acta. Roč. 180, 3-4 (2013), s. 311-318. ISSN 0026-3672. E-ISSN 1436-5073
R&D Projects: GA AV ČR(CZ) IAAX08240901
Institutional support: RVO:67179843 ; RVO:67985858
Keywords : magnetic carriers * optical fibre biosensor * diamine oxidase * fluorescence quenching * ruthenium complex
Subject RIV: EH - Ecology, Behaviour; CE - Biochemistry (UCHP-M)
Impact factor: 3.719, year: 2013

We have developed a fibre optic biosensor with incorporated magnetic microparticles for the determination of biogenic amines. The enzyme diamine oxidase from Pisum sativum was immobilized either on chitosan-coated magnetic microparticles or on commercial microbeads modified with a ferrofluid. Both the immobilized enzyme and the ruthenium complex were incorporated into a UV-cured inorganic–organic polymer composite and deposited on a lens that was connected, by optical fibres, to an electro-optical detector. The enzyme catalyzes the oxidation of amines under consumption of oxygen. The latter was determined by measuring the quenched fluorescence lifetime of the ruthenium complex. The limits of detection for the biogenic amines putrescine and cadaverine are 25–30 μmol L−1, and responses are linear up to a concentration of 1 mmol L−1.
Permanent Link: http://hdl.handle.net/11104/0217274