Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein

0380694 - ÚEB 2013 RIV DE eng J - Journal Article
Čeřovská, Noemi - Moravec, Tomáš - Plchová, Helena - Hoffmeisterová, Hana - Dědič, P.
Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein.
Journal of Phytopathology - Phytopathologische Zeitschrift. Roč. 160, č. 5 (2012), s. 251-254. ISSN 0931-1785. E-ISSN 1439-0434
R&D Projects: GA MŠMT 1M06030
Institutional research plan: CEZ:AV0Z50380511
Keywords : Potato virus M * recombinant protein * coat protein
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.000, year: 2012

The genes encoding the coat protein (CP) and triple gene block protein 1 (TGBp1) of Potato virus M (PVM) were cloned into expression vector pET-45b(+) (N-terminal 6xHis tag) and expressed in E. coli Rosetta gami-2(DE3). The purified recombinant antigens were used for raising polyclonal antibodies. The antibodies against recombinant CP were successfully used in Western blot analysis, plate-trapped ELISA and DAS-ELISA as a coating for PVM detection in infected potato leaf samples. The antibodies against recombinant non-structural protein detected the TGBp1 only in Western blot analysis. This is the first report of the production of polyclonal antibodies against recombinant coat protein and TGBp1 of PVM and their use for detecting the virus.
Permanent Link: http://hdl.handle.net/11104/0211337