0380686 - ÚEB 2013 RIV DE eng J - Journal Article
Berkman, P.J. - Skarshewski, A. - Manoli, S. - Lorenc, M. T. - Stiller, J. - Smits, L. - Lai, K. - Cambell, E. - Kubaláková, Marie - Šimková, Hana - Batley, J. - Doležel, Jaroslav - Hernandez, P. - Edwards, D.
Sequencing wheat chromosome arm 7BS delimits the 7BS/4AL translocation and reveals homoeologous gene conservation.
Theoretical and Applied Genetics. Roč. 124, č. 3 (2012), s. 423-432. ISSN 0040-5752. E-ISSN 1432-2242
R&D Projects: GA MŠMT(CZ) LC06004
Grant - others:GA MŠk(CZ) ED0007/01/01
Program: ED
Institutional research plan: CEZ:AV0Z50380511
Keywords : POLYPLOID WHEAT * BIN MAP * BRACHYPODIUM-DISTACHYON
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.658, year: 2012
DOI: https://doi.org/10.1007/s00122-011-1717-2

Complex Triticeae genomes pose a challenge to genome sequencing efforts due to their size and repetitive nature. Genome sequencing can reveal details of conservation and rearrangements between related genomes. We have applied Illumina second generation sequencing technology to sequence and assemble the low copy and unique regions of Triticum aestivum chromosome arm 7BS, followed by the construction of a syntenic build based on gene order in Brachypodium. We have delimited the position of a previously reported translocation between 7BS and 4AL with a resolution of one or a few genes and report approximately 13% genes from 7BS having been translocated to 4AL. An additional 13 genes are found on 7BS which appear to have originated from 4AL. The gene content of the 7DS and 7BS syntenic builds indicate a total of similar to 77,000 genes in wheat. Within wheat syntenic regions, 7BS and 7DS share 740 genes and a common gene conservation rate of similar to 39% of the genes from the corresponding regions in Brachypodium, as well as a common rate of colinearity with Brachypodium of similar to 60%. Comparison of wheat homoeologues revealed similar to 84% of genes previously identified in 7DS have a homoeologue on 7BS or 4AL. The conservation rates we have identified among wheat homoeologues and with Brachypodium provide a benchmark of homoeologous gene conservation levels for future comparative genomic analysis. The syntenic build of 7BS is publicly available at http://www.wheatgenome.info.

Permanent Link: http://hdl.handle.net/11104/0211330