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Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants

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    0380685 - ÚEB 2013 RIV IN eng J - Journal Article
    Čeřovská, Noemi - Hoffmeisterová, Hana - Moravec, Tomáš - Plchová, Helena - Folwarczna, Jitka - Synková, Helena - Ryšlavá, H. - Ludvíková, V. - Šmahel, M.
    Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants.
    Journal of Biosciences. Roč. 37, č. 1 (2012), s. 125-133. ISSN 0250-5991. E-ISSN 0973-7138
    R&D Projects: GA ČR GA521/06/0973; GA ČR GA521/09/1525
    Institutional research plan: CEZ:AV0Z50380511
    Keywords : Human papillomavirus (HPV-16) * L2-and E7-derived epitopes * transient expression
    Subject RIV: FD - Oncology ; Hematology
    Impact factor: 1.759, year: 2012

    Transient expression of foreign genes based on plant viral vectors is a suitable system for the production of immunogens that can be used for development of a new generation of vaccines against a variety of infectious diseases. In this study the epitope derived from HPV-16 L2 minor capsid protein (amino acids 108–120) was expressed from Potato virus X (PVX)-based vector pGR106 as N- or C-terminal fusion with the PVX coat protein (PVX CP) in transgenic Nicotiana benthamiana plants. The protein L2108-120-PVX CP was expressed in plants at a level of 170 mg/kg of fresh leaf tissue. The protein PVX CP-L2108-120 was expressed using mutated vector to avoid homologous recombination at a level of 8 mg/kg of fresh leaf tissue. Immunogenicity of L2108-120-PVX CP virus-like particles was tested after immunization of mice by subcutaneous injection or tattoo administration. In animal sera the antibodies against the PVX CP and the L2108-120 epitope were found after both methods of vaccine delivery.
    Permanent Link: http://hdl.handle.net/11104/0211329

     
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