Plant Aurora kinases play a role in maintenance of primary meristems and control of endoreduplication

0380510 - ÚEB 2013 RIV GB eng J - Journal Article
Petrovská, Beáta - Cenklová, Věra - Pochylová, Žaneta - Kourová, Hana - Doskočilová, Anna - Plíhal, Ondřej - Binarová, Lenka - Binarová, Pavla
Plant Aurora kinases play a role in maintenance of primary meristems and control of endoreduplication.
New Phytologist. Roč. 193, č. 3 (2012), s. 590-604. ISSN 0028-646X. E-ISSN 1469-8137
R&D Projects: GA ČR(CZ) GA204/07/1169; GA ČR GP204/09/P155; GA ČR(CZ) GD204/09/H084; GA MŠMT(CZ) LC06034; GA MŠMT LC545; GA AV ČR IAA500200719
Grant - others:GA MŠk(CZ) ED0007/01/01
Program: ED
Institutional research plan: CEZ:AV0Z50380511; CEZ:AV0Z50200510
Keywords : Arabidopsis thaliana * AtTPX2 (targeting protein for Xklp2) * Aurora kinases
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 6.736, year: 2012

The conserved family of Aurora kinases has multiple functions during mitosis. The roles of plant Aurora kinases have been characterized using inhibitor treatments. We down-regulated Aurora kinases in Arabidopsis thaliana using RNA interference (RNAi). We carried out a detailed phenotypic analysis of Aurora RNAi plants, biochemical and microscopic studies of AtAurora1 kinase together with AtTPX2 (targeting protein for Xklp2) and gama-tubulin. Cell division defects were observed in plants with reduced expression of Aurora kinases. Furthermore, the maintenance of primary meristems was compromised and RNAi seedlings entered endoreduplication prematurely. AtAurora1, its activator AtTPX2, and gama-tubulin were associated with microtubules in vitro; they were attached to regrowing kinetochore microtubules and colocalized on spindle microtubules and with a subset of early phragmoplast microtubules. Only the AtAurora1 kinase was translocated to the area of the cell plate. RNAi silencing of Aurora kinases showed that, in addition to their function in regulating mitosis, the kinases are required for maintaining meristematic activity and controlling the switch from meristematic cell proliferation to differentiation and endoreduplication. The colocalization and co-fractionation of AtAurora1 with AtTPX2, and gama-tubulin on microtubules in a cell cycle-specific manner suggests that AtAurora1 kinase may function to phosphorylate substrates that are critical to the spatiotemporal regulation of acentrosomal microtubule formation and organization.
Permanent Link: http://hdl.handle.net/11104/0211200