Patterns of Early Gut Colonization Shape Future Immune Responses of the Host

0378860 - MBÚ 2013 RIV US eng J - Journal Article
Hansen, C. H. F. - Nielsen, D. S. - Kverka, Miloslav - Zákostelská, Zuzana - Klimešová, Klára - Hudcovic, Tomáš - Tlaskalová-Hogenová, Helena - Hansen, A. K.
Patterns of Early Gut Colonization Shape Future Immune Responses of the Host.
PLoS ONE. Roč. 7, č. 3 (2012), e34043. ISSN 1932-6203. E-ISSN 1932-6203
Institutional research plan: CEZ:AV0Z50200510
Keywords : SEGMENTED FILAMENTOUS BACTERIA * REGULATORY T-CELLS * MICROBIOTA
Subject RIV: EC - Immunology
Impact factor: 3.730, year: 2012

The most important trigger for immune system development is the exposure to microbial components immediately after birth. Moreover, targeted manipulation of the microbiota can be used to change host susceptibility to immune-mediated diseases. Our aim was to analyze how differences in early gut colonization patterns change the composition of the resident microbiota and future immune system reactivity. Germ-free (GF) mice were either inoculated by single oral gavage of caecal content or let colonized by co-housing with specific pathogen-free (SPF) mice at different time points in the postnatal period. The microbiota composition was analyzed by denaturing gradient gel electrophoresis for 16S rRNA gene followed by principal component analysis
Permanent Link: http://hdl.handle.net/11104/0210195