In Vivo Determination of Organellar pH Using a Universal Wavelength-Based Confocal Microscopy Approach

0377982 - MBÚ 2013 RIV US eng J - Journal Article
Rodó, A. P. - Váchová, Libuše - Palková, Z.
In Vivo Determination of Organellar pH Using a Universal Wavelength-Based Confocal Microscopy Approach.
PLoS ONE. Roč. 7, č. 3 (2012), s. 1-12. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA ČR GA204/08/0718
Institutional research plan: CEZ:AV0Z50200510
Keywords : GREEN FLUORESCENT PROTEIN * YEAST COLONY DEVELOPMENT * INTRACELLULAR PH
Subject RIV: EE - Microbiology, Virology
Impact factor: 3.730, year: 2012

Many essential cellular processes are affected by transmembrane H+ gradients and intracellular pH (pHi). The research of such metabolic events calls for a non-invasive method to monitor pHi within individual subcellular compartments. We present a novel confocal microscopy approach for the determination of organellar pHi in living cells expressing pHdependent ratiometric fluorescent proteins. Unlike conventional intensity-based fluorometry, our method relies on emission wavelength scans at single-organelle resolution to produce wavelength-based pH estimates both accurate and robust to low-signal artifacts
Permanent Link: http://hdl.handle.net/11104/0209983