Spatial Distribution of Glycerophospholipids in the Ocular Lens

0371571 - MBÚ 2012 RIV US eng J - Journal Article
Pól, Jaroslav - Vidová, Veronika - Hyötyläinen, T. - Volný, Michael - Novák, Petr - Strohalm, Martin - Kostiainen, R. - Havlíček, Vladimír - Wiedmer, S. K. - Holopainen, J.
Spatial Distribution of Glycerophospholipids in the Ocular Lens.
PLoS ONE. Roč. 6, č. 4 (2011), s. 1-9. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA MŠMT LC07017; GA ČR GPP206/10/P018
Institutional research plan: CEZ:AV0Z50200510
Keywords : MASS SPECTROMETRY * MEMBRANE-PROTEINS * ALPHA-CRYSTALLIN
Subject RIV: CE - Biochemistry
Impact factor: 4.092, year: 2011

Knowledge of the spatial distribution of lipids in the intraocular lens is important for understanding the physiology and biochemistry of this unique tissue and for gaining a better insight into the mechanisms underlying diseases of the lens. Following our previous study showing the spatial distribution of sphingolipids in the porcine lens, the current study used ultra performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOFMS) to provide the whole lipidome of porcine lens and these studies were supplemented by matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) of the lens using ultra-high resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) to determine the spatial distribution of glycerophospholipids
Permanent Link: http://hdl.handle.net/11104/0205056