Chromosome Isolation by Flow Sorting in Aegilops umbellulata and Ae. comosa and Their Allotetraploid Hybrids Ae. biuncialis and Ae. geniculata

0368586 - ÚEB 2012 RIV US eng J - Journal Article
Molnár, I. - Kubaláková, Marie - Šimková, Hana - Cseh, A. - Molnár-Láng, M. - Doležel, Jaroslav
Chromosome Isolation by Flow Sorting in Aegilops umbellulata and Ae. comosa and Their Allotetraploid Hybrids Ae. biuncialis and Ae. geniculata.
PLoS ONE. Roč. 66, č. 11 (2011), s. 1-11. ISSN 1932-6203. E-ISSN 1932-6203
R&D Projects: GA MŠMT(CZ) LC06004; GA MŠMT OC08025
Grant - others:European Regional Development Fund CZ.1.05/2.1.00/ 01.0007
Institutional research plan: CEZ:AV0Z50380511
Keywords : wheat * chromosome isolation * Aegilops umbellulata
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 4.092, year: 2011

This study evaluates the potential of flow cytometry for chromosome sorting in two wild diploid wheats Aegilops umbellulata and Ae. comosa and their natural allotetraploid hybrids Ae. biuncialis and Ae. geniculata. Flow karyotypes obtained after the analysis of DAPI-stained chromosomes were characterized and content of chromosome peaks was determined. Peaks of chromosome 1U could be discriminated in flow karyotypes of Ae. umbellulata and Ae. biuncialis and the chromosome could be sorted with purities exceeding 95%. The remaining chromosomes formed composite peaks and could be sorted in groups of two to four. Twenty four wheat SSR markers were tested for their position on chromosomes of Ae. umbellulata and Ae. comosa using PCR on DNA amplified from flow-sorted chromosomes and genomic DNA of wheat-Ae. geniculata addition lines, respectively. Six SSR markers were located on particular Aegilops chromosomes using sorted chromosomes, thus confirming the usefulness of this approach for physical mapping.
Permanent Link: http://hdl.handle.net/11104/0202886