Genomic constitution of Festuca x Lolium hybrids revealed by the DArTFest array

0364120 - ÚEB 2012 RIV DE eng J - Journal Article
Kopecký, David - Bartoš, Jan - Christelová, Pavla - Černoch, V. - Kilian, A. - Doležel, Jaroslav
Genomic constitution of Festuca x Lolium hybrids revealed by the DArTFest array.
Theoretical and Applied Genetics. Roč. 122, č. 2 (2011), s. 355-363. ISSN 0040-5752. E-ISSN 1432-2242
R&D Projects: GA MZe QH71267; GA ČR GP521/07/P479
Institutional research plan: CEZ:AV0Z50380511
Keywords : IN-SITU HYBRIDIZATION * GENETIC-LINKAGE MAP * PRATENSIS HUDS
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 3.297, year: 2011

Complementary attributes of Festuca and Lolium grasses can be combined in hybrid cultivars called Festuloliums, which are becoming increasingly popular fodder crops and amenity plants. Genomic constitution of commercially available Festuloliums was reported to vary from almost equal representation of parental genomes to apparent lack of one of them based on molecular cytogenetic analyses and screening with a small set of DNA markers, both approaches with limited resolution. Here, we describe the use of the DArTFest array comprising 3,884 polymorphic DArT markers for characterization of genomes in five Festulolium cultivars. Using the DArTFest array, it was possible to determine hybrid genome constitution at resolution which has never been achieved before and the analysis of a set of randomly selected plants from each cultivar provided information on genetic structure of outcrossing Festulolium cultivars.
Permanent Link: http://hdl.handle.net/11104/0199686