Role of hydrogen peroxide and antioxidant enzymes in the interaction between a hemibiotrophic fungal pathogen, Leptosphaeria maculans, and oilseed rape

0360027 - ÚEB 2012 RIV NL eng J - Journal Article
Jindřichová, Barbora - Fodor, J. - Šindelářová, Milada - Burketová, Lenka - Valentová, O.
Role of hydrogen peroxide and antioxidant enzymes in the interaction between a hemibiotrophic fungal pathogen, Leptosphaeria maculans, and oilseed rape.
Environmental and Experimental Botany. Roč. 72, č. 2 (2011), s. 149-156. ISSN 0098-8472. E-ISSN 1873-7307
R&D Projects: GA ČR GA522/08/1581; GA MŠMT MEB040923
Institutional research plan: CEZ:AV0Z50380511
Keywords : hydrogen peroxide * antioxidant enzymes * hemibiotrophic pathogen
Subject RIV: GF - Plant Pathology, Vermin, Weed, Plant Protection
Impact factor: 2.985, year: 2011

Reactive oxygen species play a dual role in host–pathogen interaction. They impede the spread of biotrophic pathogens via stimulating cell death and hypersensitive response (HR), and, on the other hand, they provide access to nutrients for necrotrophic pathogens feeding on dead tissues and facilitate their colonizing the host. The participation of ROS in defending plants from pathogens with a combined lifestyle (hemibiotrophs) is not yet understood, and it varies in its dependence on the particular host–pathogen combination. In the present study, we inoculated rapeseed plants (Brassica napus) with a hemibiotrophic fungus, Leptosphaeria maculans, and manipulated the H2O2 content in cotyledons by infiltrating catalase and/or H2O2 into tissues. The action of catalase resulted in a significant decrease in lesions development, but when H2O2 was applied instead, lesion formation was only moderately stimulated compared to the untreated control.
Permanent Link: http://hdl.handle.net/11104/0197678