Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures

0357035 - ÚEB 2011 RIV GB eng J - Journal Article
Vondráková, Zuzana - Cvikrová, Milena - Eliášová, Kateřina - Martincová, Olga - Vágner, Martin
Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures.
Tree Physiology. Roč. 30, č. 10 (2010), s. 1335-1348. ISSN 0829-318X. E-ISSN 1758-4469
R&D Projects: GA MŠMT OC 158; GA MŠMT OC08013
Institutional research plan: CEZ:AV0Z50380511
Keywords : cryopreservation * Norway spruce (Picea abies (L ) Karst) * somatic embryogenesis
Subject RIV: EA - Cell Biology
Impact factor: 2.403, year: 2010

The anatomical characteristics and PA content during cryopreservation and regrowth were studied in the ESMs of AFO 541 and C110 cultures, which have comparable ESM anatomy but diverse growth rates, PA content and regeneration abilities after cryopreservation. The endogenous free PAs were maintained at high levels, with spermidine being the predominant PA in the AFO 541, while in C110 the content of putrescine and spermidine was almost identical and rather low, the content of spermidine being approximately one-third that in the ESM of AFO 541. Osmotic pretreatment, using a double application of sorbitol followed by an application of dimethyl sulfoxide (DMSO) resulted in the continual disintegration of polyembryogenic centers and suspensors in both cell lines. A continual decrease in the level of PAs was observed during the cell osmotic pretreatment. The cells that retained their viability and regrowth ability after cryopreservation were the meristematic cells inside embryonal heads.
Permanent Link: http://hdl.handle.net/11104/0195407