Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds

0348486 - ÚEB 2011 RIV NL eng J - Journal Article
Semenyuk, E.G. - Schmidt, M.A. - Beachy, R.N. - Moravec, Tomáš - Woodford-Thomas, T.
Adaptation of an ecdysone-based genetic switch for transgene expression in soybean seeds.
Transgenic Research. Roč. 19, č. 6 (2010), s. 987-999. ISSN 0962-8819. E-ISSN 1573-9368
Institutional research plan: CEZ:AV0Z50380511
Keywords : Inducible expression * Soybean * Seed-specific genes
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.569, year: 2010

Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFPKDEL reporter protein upon addition of the chemical ligand, methoxyfenozide. The chemical gene switch activated gene expression under in vitro conditions in somatic cotyledonary embryos and zygotic seed embryos cultured from transgenic soybean plants, as well as in seeds in planta under greenhouse conditions. The efficiency of induction of GFP expression under different growth conditions was strongly influenced by the developmental stage of the seed and availability of the inducer. The formation of ER-derived GFP-containing protein bodies in seed storage parenchyma cells was correlated with the level of induced expression.
Permanent Link: http://hdl.handle.net/11104/0189013