Production of Polyclonal Antibodies to Potato virus X Using Recombinant Coat Protein

0347697 - ÚEB 2011 RIV DE eng J - Journal Article
Čeřovská, Noemi - Moravec, Tomáš - Plchová, Helena - Hoffmeisterová, Hana - Kmoníčková, Jitka - Dědič, P.
Production of Polyclonal Antibodies to Potato virus X Using Recombinant Coat Protein.
Journal of Phytopathology - Phytopathologische Zeitschrift. Roč. 158, č. 1 (2010), s. 66-68. ISSN 0931-1785. E-ISSN 1439-0434
R&D Projects: GA MŠMT 1M06030
Institutional research plan: CEZ:AV0Z50380511
Keywords : Potato virus X * recombinant viral antigen * antibodies
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.937, year: 2010

Polyclonal antibodies to recombinant Potato virus X (PVX) coat protein (PVX-CP) were developed and their effectiveness determined in different ELISA protocols. The PVX-CP gene was amplified by reverse transcription-polymerase chain reaction, cloned and expressed in Escherichia coli. For immunization, the CP fractions from bacterial lysate were purified either by simple fractionation or by excision from sodium dodecyl sulphate gels. The PVX-CP was injected into rabbits for antibody production. The PVX-CP antibodies reacted in an indirect plate trapped antigen enzyme-linked immunosorbent assay and immunoblot assay and were useful for the detection of a broad spectrum of isolates of PVX.
Permanent Link: http://hdl.handle.net/11104/0188422