Lipoxygenase inhibitors enhance tumor suppressive effects of Jun proteins v-myb-transformed monoblasts BM2

0127049 - BFU-R 20033137 RIV US eng J - Journal Article
Bryja, V. - Sedláček, J. - Zahradníčková, E. - Ševčíková, S. - Pacherník, Jiří - Souček, Karel - Hofmanová, Jiřina - Kozubík, Alois - Šmarda, J.
Lipoxygenase inhibitors enhance tumor suppressive effects of Jun proteins v-myb-transformed monoblasts BM2.
Prostaglandins. Roč. 72, 3-4 (2003), s. 131-145. ISSN 0090-6980
R&D Projects: GA ČR GA301/01/0040; GA ČR GA524/03/0766
Institutional research plan: CEZ:AV0Z5004920
Keywords : hematopoiesis * arachidonic acid * lipoxygenase inhibitors
Subject RIV: BO - Biophysics
Impact factor: 2.594, year: 2003

Inhibitors of arachidonic acid (AA) conversion were described as suppressors of proliferation and inducers of differentiation of various leukemic cells. Certain AA metabolites have been shown to cooperate with Jun proteins that are important factors controlling cell proliferation, differentiation and apoptosis. Using lipoxygenase (LOX) inhibitors of various specifity we studied possible participation of lipoxygenase pathway in regulation of proliferation and apoptosis of v-myb-transformed chicken monoblasts BM2 and its functional interaction with Jun proteins. We found that nordihydroguaiaretic acid (NDGA) and esculetin (Esc) negatively regulate proliferation of BM2 cells causing accumulation in either G(0)/G(1)-phase (nordihydroguaiaretic acid) or S-phase (esculetin) of the cell cycle. BM2 cells can be also induced to undergo growth arrest and partial differentiation by ectopic expression of Jun proteins.
Permanent Link: http://hdl.handle.net/11104/0025273