0635813 - ÚEB 2026 RIV DE eng J - Journal Article
Korchanová, Zuzana - Milovanov, A. - Švec, M. - Doležel, Jaroslav - Bartoš, Jan - Valárik, Miroslav
Progress and innovations of gene cloning in wheat and its close relatives.
Theoretical and Applied Genetics. Roč. 138, č. 5 (2025), č. článku 106. ISSN 0040-5752. E-ISSN 1432-2242
R&D Projects: GA MŠMT(CZ) EH22_008/0004581; GA MZe(CZ) QK22010293
Institutional support: RVO:61389030
Keywords : powdery mildew resistance * triticum-aestivum l. * chromosome bac library * map-based isolation * bread wheat * aegilops-tauschii * conferring resistance * genome sequence * grain protein * linkage maps
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 4.4, year: 2023 ; AIS: 0.919, rok: 2023
Method of publishing: Open access
Result website:
https://doi.org/10.1007/s00122-025-04897-wDOI: https://doi.org/10.1007/s00122-025-04897-w

The large and complex genomes of cereals, especially bread wheat, have always been a challenge for gene mapping and cloning. Nevertheless, recent advances in genomics have led to significant progress in this field. Currently, high-quality reference sequences are available for major wheat species and their relatives. New high-throughput genotyping platforms and next-generation sequencing technologies combined with genome complexity reduction techniques and mutagenesis have opened new avenues for gene cloning. In this review, we provide a comprehensive overview of the genes cloned in wheat so far and discuss the strategies used for cloning these genes. We highlight the advantages and drawbacks of individual approaches and show how particular genomic progress contributed to wheat gene cloning. A wide range of new resources and approaches have led to a significant increase in the number of successful cloning projects over the past decade, demonstrating that it is now feasible to perform rapid gene cloning of agronomically important genes, even in a genome as large and complex as that of wheat.
Permanent Link: https://hdl.handle.net/11104/0366837