0635671 - ÚOCHB 2026 RIV US eng J - Journal Article
Šinkevičiūtė, Ugnė - Sánchez Quirante, Tania - Rožánková, Samanta - Poštová Slavětínská, Lenka - Raindlová, Veronika - Hocek, Michal
Synthesis of 2-Substituted Adenosine Triphosphate Derivatives and their use in Enzymatic Synthesis and Postsynthetic Labelling of RNA.
Chembiochem. Roč. 26, č. 12 (2025), č. článku e202500241. ISSN 1439-4227. E-ISSN 1439-7633
R&D Projects: GA MŠMT(CZ) EH22_008/0004575
EU Projects: European Commission(XE) 861381 - NATURE-ETN
Institutional support: RVO:61388963
Keywords : click reactions * DNA polymerases * nucleosides triphosphates * nucleotides * polyA polymerase * RNA polymerases * thiol-ene addition
Impact factor: 2.6, year: 2023 ; AIS: 0.736, rok: 2023
Method of publishing: Open access
Result website:
https://doi.org/10.1002/cbic.202500241DOI: https://doi.org/10.1002/cbic.202500241

A series of adenosine triphosphate (ATP) derivatives bearing chloro, fluoro, amino, methyl, vinyl, and ethynyl groups at position 2 are synthesized and tested as substrates for RNA and DNA polymerases. The modified nucleotides work well in in vitro transcription with T7 RNA polymerase and primer extension (PEX) using engineered DNA polymerases (TGK, 2M) except for the bulkier 2-vinyl- and 2-ethynyl-ATP derivatives that give truncated products. However, in single nucleotide incorporation followed by PEX, they still can be used for site-specific incorporation of reactive modifications into RNA that can be further used for postsynthetic labeling through thiol-ene or Cu-catalyzed alkyne-azide cycloadditions reactions. All modified ATPs work in polyadenylation catalyzed by poly(A) polymerase to form long 3 '-polyA tails containing the modifications that also can be used for labeling.
Permanent Link: https://hdl.handle.net/11104/0366704
Research data: Národní repozitář