0627729 - BFÚ 2026 RIV NL eng J - Journal Article
Hesko, Ondřej - Pivoňková, Hana - Fojt, Lukáš - Taylor, Andrew - Kopeček, Jaromír - Schwarzová-Pecková, K. - Fojta, Miroslav
Characterization of boron doped diamond electrodes with engineered sp2 carbon content and their application to structure-dependent DNA hybridization.
Bioelectrochemistry. Roč. 164, AUG 2025 (2025), č. článku 108910. ISSN 1567-5394. E-ISSN 1878-562X
R&D Projects: GA ČR(CZ) GA23-05688S; GA MŠMT LM2023051; GA MŠMT EF15_003/0000477
Institutional support: RVO:68081707 ; RVO:68378271
Keywords : transfer kinetics * g-quadruplexes * doping level * surface * morphology * electrochemistry * impact * bases
OECD category: Biochemistry and molecular biology; Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) (FZU-D)
Impact factor: 4.8, year: 2023 ; AIS: 0.727, rok: 2023
Method of publishing: Limited access
Result website:
https://www.sciencedirect.com/science/article/pii/S1567539425000131?via%3DihubDOI: https://doi.org/10.1016/j.bioelechem.2025.108910

Boron doped diamond electrodes brought a new potential in bioanalytical chemistry including studies of structure and interactions of nucleic acids. Herein, deposition conditions were optimized to produce a set of polycrystalline BDD electrodes with comparable boron concentration in solid phase of (1.8 2.1) <middle dot> 10(21) cm(-3) akin to metallic-type conductivity but with increasing sp(2) carbon content. Increase of [CH4]/[H-2] from 0.25 % to 2.0 % during deposition led to an obvious decrease in grain size from ca. 300 nm (BDD0.25) to < 100 nm (BDD2.0). Adsorption of oligodeoxynucleotides and their structural changes in the presence of K+ and Li+ ions were evaluated through enzyme-linked DNA hybridization assay in which oxidizable 1-naphthol was released from its phosphoester by streptavidin-alkaline phosphatase conjugate upon successful hybridization of the target oligodeoxynucleotide with a biotinylated complementary probe. With increasing sp(2) carbon content, the hybridization assay showed improved discrimination between a target forming guanine quadruplex (stabilized by K+ ions), yielding by 40 % 60 % lower hybridization signal with the complementary probe, compared to the same but unstructured target oligodeoxynucleotide in the presence of Li+ ions that don't stabilize the quadruplex structure. Such behaviour was observed also for commercial BDD electrode with surface roughness < 10 nm.
Permanent Link: https://hdl.handle.net/11104/0366521