0618720 - FZÚ 2026 RIV GB eng J - Journal Article
Elblová, Petra - Andělová, Hana - Lunova, Mariia - Anthi, Judita - Henry, S.J.W. - Tu, X.Y. - Dejneka, Alexandr - Jirsa, M. - Stephanopoulos, N. - Lunov, Oleg
Geometrically constrained cytoskeletal reorganisation modulates DNA nanostructures uptake.
Journal of Materials Chemistry B. Roč. 13, č. 7 (2025), s. 2335-2351. ISSN 2050-750X. E-ISSN 2050-7518
R&D Projects: GA MŠMT(CZ) LUAUS24038
Institutional support: RVO:68378271
Keywords : DNA nanotechnology * nanotechnology * cytoskeletal reorganisation * nanoparticle uptake
OECD category: Biophysics
Impact factor: 6.1, year: 2023 ; AIS: 0.952, rok: 2023
Method of publishing: Open access
DOI: https://doi.org/10.1039/D5TB00074B

DNA nanostructures (DNs) have gained popularity in various biomedical applications due to their unique properties, including structural programmability, ease of synthesis and functionalization, and low cytotoxicity. Effective utilization of DNs in biomedical applications requires a fundamental understanding of their interactions with living cells and the mechanics of cellular uptake. Current knowledge primarily focuses on how the physicochemical properties of DNs, such as mass, shape, size, and surface functionalization, affect uptake efficacy. However, the role of cellular mechanics and morphology in DN uptake remains largely unexplored. In this work, we show that cells subjected to geometric constraints remodel their actin cytoskeleton, resulting in differential mechanical force generation that facilitates DN uptake. The length, number, and orientation of F-actin fibers are influenced by these constraints, leading to distinct mechanophenotypes. Overall, DN uptake is governed by F-actin forces arising from filament reorganisation under geometric constraints. These results underscore the importance of actin dynamics in the cellular uptake of DNs and suggest that leveraging geometric constraints to induce specific cell morphology adaptations could enhance the uptake of therapeutically designed DNs.
Permanent Link: https://hdl.handle.net/11104/0365605