0601978 - ÚEB 2025 RIV US eng J - Journal Article
Oklešťková, Jana - Kvasnica, Miroslav - Strnad, Miroslav
Analytical Methods for Brassinosteroid Analysis: Recent Advances and Applications.
Plant and Cell Physiology. Roč. 65, č. 10 (2024), s. 1618-1626. ISSN 0032-0781. E-ISSN 1471-9053
Institutional support: RVO:61389030
Keywords : solid-phase extraction * endogenous brassinosteroids * mass-spectrometry * boronate affinity * different tissues * plant * quantification * brassinolide * castasterone * sensitivity * Brassinosteroids * Chemical synthesis * Chromatography * Immunoassays * Mass spectrometry * Quantification
OECD category: Cell biology
Impact factor: 4, year: 2023 ; AIS: 1.084, rok: 2023
Method of publishing: Open access
Result website:
https://doi.org/10.1093/pcp/pcae038DOI: https://doi.org/10.1093/pcp/pcae038

Brassinosteroids (BRs) are plant steroidal hormones that play crucial roles in plant growth and development. Accurate quantification of BRs in plant tissues is essential for understanding their biological functions. This study presents a comprehensive overview of the latest methods used for the quantification of BRs in plants. We discuss the principles, advantages and limitations of various analytical techniques, including immunoassays, gas chromatography-mass spectrometry and liquid chromatography-tandem mass spectrometry that are used for the detection and quantification of BRs from complex plant matrixes. We also explore the use of isotopically labeled internal standards to improve the accuracy and reliability of BR quantification.
Permanent Link: https://hdl.handle.net/11104/0359202