0601027 - ÚACH 2025 RIV DE eng J - Journal Article
Pereira, L. V. - Rizzi, T. - Federizzi, M. - Donato, Ricardo Keitel - Fuentefria, A. M. - Reginatto, P. … Total 7 authors
Antifungal Associations with a Polyelectrolyte Promote Significant Reduction of Minimum Inhibitory Concentrations against Opportunistic Candida spp. Strains.
Current Microbiology. Roč. 81, č. 12 (2024), č. článku 441. ISSN 0343-8651. E-ISSN 1432-0991
Institutional support: RVO:61388980
Keywords : Amphotericin B * Antifungal Agents * Candida * Drug Synergism * Microbial Sensitivity Tests * Pichia * Quaternary Ammonium Compounds
OECD category: Inorganic and nuclear chemistry
Impact factor: 2.3, year: 2023 ; AIS: 0.446, rok: 2023
Method of publishing: Limited access
Result website:
https://doi.org/10.1007/s00284-024-03960-xDOI: https://doi.org/10.1007/s00284-024-03960-x

The current global scenario presents us with a growing increase in infections caused by fungi, referred to by specialists in the field as a “silent epidemic”, aggravated by the limited pharmacological arsenal and increasing resistance to this therapy. For this reason, drug repositioning and therapeutic compound combinations are promising strategies to mitigate this serious problem. In this context, this study investigates the antifungal activity of the non-toxic, low-cost and widely available cationic polyelectrolyte Poly(diallyldimethylammonium chloride) (PDDA), in combination with different antifungal drugs: systemic (amphotericin B, AMB), topical (clioquinol, CLIO) and oral (nitroxoline, NTX). For each combination, different drug:PDDA ratios were tested and, through the broth microdilution technique, the minimum inhibitory concentration (MIC) of these drugs in the different ratios against clinically important Candida species strains was determined. Overall, PDDA combinations with the studied drugs demonstrated a significant increase in drug activity against most strains, reaching MIC reductions of up to 512 fold for the fluconazole resistant Candida krusei (Pichia kudriavzevii). In particular, the AMB-PDDA combination 1:99 was highly effective against AMB-resistant strains, demonstrating the excellent profile of PDDA as an adjuvant/association in novel antifungal formulations with outdated conventional drugs.
Permanent Link: https://hdl.handle.net/11104/0358509