0599837 - ÚEB 2025 RIV US eng J - Journal Article
Kaleta, Michal - Oklešťková, Jana - Klíčová, K. - Kvasnica, Miroslav - Koníčková, D. - Menšíková, K. - Strnad, Miroslav - Novák, Ondřej
Simultaneous Determination of Selected Steroids with Neuroactive Effects in Human Serum by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry.
ACS Chemical Neuroscience. Roč. 15, č. 10 (2024), s. 1990-2005. ISSN 1948-7193. E-ISSN 1948-7193
Institutional support: RVO:61389030
Keywords : traumatic brain-injury * solid-phase extraction * cerebrospinal-fluid * progesterone metabolite * endogenous steroids * human plasma * human saliva * uhplc-ms/ms * dehydroepiandrosterone * neurosteroids * neuroactive steroids * neurosteroids * ultrahigh-performanceliquid chromatography-tandem mass spectrometry * serum
OECD category: Biochemistry and molecular biology
Impact factor: 4.1, year: 2023 ; AIS: 0.975, rok: 2023
Method of publishing: Open access
Result website:
https://doi.org/10.1021/acschemneuro.3c00824DOI: https://doi.org/10.1021/acschemneuro.3c00824

Neuroactive steroids are a group of steroid molecules that are involved in the regulation of functions of the nervous system. The nervous system is not only the site of their action, but their biosynthesis can also occur there. Neuroactive steroid levels depend not only on the physiological state of an individual (person's sex, age, diurnal variation, etc.), but they are also affected by various pathological processes in the nervous system (some neurological and psychiatric diseases or injuries), and new knowledge can be gained by monitoring these processes. The aim of our research was to develop and validate a comprehensive method for the simultaneous determination of selected steroids with neuroactive effects in human serum. The developed method enables high throughput and a sensitive quantitative analysis of nine neuroactive steroid substances (pregnenolone, progesterone, 5 alpha-dihydroprogesterone, allopregnanolone, testosterone, 5 alpha-dihydrotestosterone, androstenedione, dehydroepiandrosterone, and epiandrosterone) in 150 mu L of human serum by ultrahigh-performance liquid chromatography with tandem mass spectrometry. The correlation coefficients above 0.999 indicated that the developed analytical procedure was linear in the range of 0.90 nmol/L to 28.46 mu mol/L in human serum. The accuracy and precision of the method for all analytes ranged from 83 to 118% and from 0.9 to 14.1%, respectively. This described method could contribute to a deeper understanding of the pathophysiology of various diseases. Similarly, it can also be helpful in the search for new biomarkers and diagnostic options or therapeutic approaches.
Permanent Link: https://hdl.handle.net/11104/0357265