0599487 - ÚIACH 2025 RIV IT eng A - Abstract
Smolková, Denisa - Duša, Filip - Šesták, Jozef - Čmelík, Richard - Lavická, Jana
Capillary electrophoresis analysis of oligosaccharides and glycans using rhodamine B-based labeling.
ISSS 2024 : Book of Abstract. 2024. s. 102.
[ISSS 2024. International Symposium on Separation Sciences /28./. 22.09.2024-25.09.2024, Messina]
R&D Projects: GA MŠMT(CZ) 9F23002; GA ČR(CZ) GA22-00236S
Institutional support: RVO:68081715
Keywords : glycan analysis * labeling * capillary electrophoresis * fluorescence
OECD category: Analytical chemistry
Result website:
https://www.sepscisoc.com/isss2024

We successfully designed and synthesized a novel fluorescent rhodamine B-based label for CE analysis of oligosaccharides and glycans. We optimized labeling conditions to ensure the most efficient derivatization, including reaction temperature and time, solvent composition, and separation parameters. Additionally, the rhodamine B labeling method was employed for N-linked glycan profiling of several glycoproteins using CE/LIF analysis.
Permanent Link: https://hdl.handle.net/11104/0356924