0599397 - ÚVGZ 2025 RIV US eng J - Journal Article
Pfennig, T. - Kullmann, E. - Zavřel, Tomáš - Nakielski, A. - Ebenhoeh, O. - Červený, Jan - Bernát, G. - Matuszynska, A. B.
Shedding light on blue-green photosynthesis: A wavelength-dependent mathematical model of photosynthesis in iSynechocystis/i sp. PCC 6803.
PLoS Computational Biology. Roč. 20, č. 9 (2024), č. článku e1012445. ISSN 1553-734X. E-ISSN 1553-7358
R&D Projects: GA MŠMT(CZ) LM2018123; GA MŠMT(CZ) EF16_026/0008413; GA MŠMT(CZ) LUAUS24149
Institutional support: RVO:86652079
Keywords : orange carotenoid protein * electron-transport * cyanobacteria * quality * energy * transitions * pathways * pcc-6803 * co2
OECD category: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impact factor: 3.8, year: 2023 ; AIS: 1.831, rok: 2023
Method of publishing: Open access
Result website:
https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1012445DOI: https://doi.org/10.1371/journal.pcbi.1012445

Cyanobacteria hold great potential to revolutionize conventional industries and farming practices with their light-driven chemical production. To fully exploit their photosynthetic capacity and enhance product yield, it is crucial to investigate their intricate interplay with the environment including the light intensity and spectrum. Mathematical models provide valuable insights for optimizing strategies in this pursuit. In this study, we present an ordinary differential equation-based model for the cyanobacterium Synechocystis sp. PCC 6803 to assess its performance under various light sources, including monochromatic light. Our model can reproduce a variety of physiologically measured quantities, e.g. experimentally reported partitioning of electrons through four main pathways, O-2 evolution, and the rate of carbon fixation for ambient and saturated CO2. By capturing the interactions between different components of a photosynthetic system, our model helps in understanding the underlying mechanisms driving system behavior. Our model qualitatively reproduces fluorescence emitted under various light regimes, replicating Pulse-amplitude modulation (PAM) fluorometry experiments with saturating pulses. Using our model, we test four hypothesized mechanisms of cyanobacterial state transitions for ensemble of parameter sets and found no physiological benefit of a model assuming phycobilisome detachment. Moreover, we evaluate metabolic control for biotechnological production under diverse light colors and irradiances. We suggest gene targets for overexpression under different illuminations to increase the yield. By offering a comprehensive computational model of cyanobacterial photosynthesis, our work enhances the basic understanding of light-dependent cyanobacterial behavior and sets the first wavelength-dependent framework to systematically test their producing capacity for biocatalysis.
Permanent Link: https://hdl.handle.net/11104/0356867