0598552 - BTÚ 2025 RIV US eng J - Journal Article
Krattenmacher, Jochen - Lera-Ramirez, M. - Beber, Alexandre - Herynek, Štěpán - Gryčová, Lenka - Liu, X. - Neuzil, P. - Nedelec, F. - Diez, S. - Braun, Marcus - Lánský, Zdeněk
Ase1 selectively increases the lifetime of antiparallel microtubule overlaps.
Current Biology. Roč. 34, č. 17 (2024). ISSN 0960-9822. E-ISSN 1879-0445
R&D Projects: GA ČR(CZ) GX19-27477X; GA ČR(CZ) GA22-11753S; GA ČR(CZ) GA19-17398S; GA MŠMT(CZ) LX22NPO5107; GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_013/0001775
Research Infrastructure: CIISB III - 90242
Institutional support: RVO:86652036
Keywords : cross-linking * driven * prc1 * depolymerization * purification * tubulin * arrays * length
OECD category: Biochemistry and molecular biology
Impact factor: 8.1, year: 2023 ; AIS: 4.38, rok: 2023
Method of publishing: Open access
Result website:
https://www.sciencedirect.com/science/article/pii/S0960982224009941?via%3DihubDOI: https://doi.org/10.1016/j.cub.2024.07.055

Microtubules (MTs) are dynamically unstable polar biopolymers switching between periods of polymerization and depolymerization, with the switch from the polymerization to the depolymerization phase termed catastrophe and the reverse transition termed rescue.1 1 In presence of MT-crosslinking proteins, MTs form parallel or anti-parallel overlaps and self-assemble reversibly into complex networks, such as the mitotic spindle. Differential regulation of MT dynamics in parallel and anti-parallel overlaps is critical for the self-assembly of these networks.2,3 2,3 Diffusible MT crosslinkers of the Ase1/MAP65/PRC1 family associate with different affinities to parallel and antiparallel MT overlaps, providing a basis for this differential regulation.4-11 4-11 Ase1/ MAP65/PRC1 family proteins directly affect MT dynamics12 12 and recruit other proteins that locally alter MT dynamics, such as CLASP or kinesin-4. (7,13-16 )However, how Ase1 differentially regulates MT stability in parallel and antiparallel bundles is unknown. Here, we show that Ase1 selectively promotes antiparallel MT overlap longevity by slowing down the depolymerization velocity and by increasing the rescue frequency, specifically in antiparallelly crosslinked MTs. At the retracting ends of depolymerizing MTs, concomitant with slower depolymerization, we observe retention and accumulation of Ase1 between crosslinked MTs and on isolated MTs. We hypothesize that the ability of Ase1 to reduce the dissociation of tubulin subunits is sufficient to promote its enrichment at MT ends. A mathematical model built on this idea shows good agreement with the experiments. We propose that differential regulation of MT dynamics by Ase1 contributes to mitotic spindle assembly by specifically stabilizing antiparallel overlaps, compared to parallel overlaps or isolated MTs.
Permanent Link: https://hdl.handle.net/11104/0356410