0598195 - ÚMCH 2025 RIV US eng J - Journal Article
Hlukhaniuk, Anna - Swietek, Malgorzata Anna - Patsula, Vitalii - Hodan, Jiří - Janoušková, O. - Bystrianský, L. - Brož, Antonín - Malić, Marina - Zasońska, Beata Anna - Tokarz, W. - Bačáková, Lucie - Horák, Daniel
Poly(epsilon-caprolactone)-based composites modified with polymer-grafted magnetic nanoparticles and L-ascorbic acid for bone tissue engineering.
Journal of Biomedical Materials Research. Part B. Roč. 112, č. 9 (2024), č. článku e35480. ISSN 1552-4973. E-ISSN 1552-4981
R&D Projects: GA ČR(CZ) GA20-07015S; GA MŠMT LX22NPO5102
Institutional support: RVO:61389013 ; RVO:67985823
Keywords : iron oxide nanoparticles * L-ascorbic acid * nanocomposites
OECD category: Composites (including laminates, reinforced plastics, cermets, combined natural and synthetic fibre fabrics; Cell biology (FGU-C)
Impact factor: 3.2, year: 2023 ; AIS: 0.485, rok: 2023
Method of publishing: Open access
Result website:
https://onlinelibrary.wiley.com/doi/10.1002/jbm.b.35480DOI: https://doi.org/10.1002/jbm.b.35480

The aim of this study was to develop multifunctional magnetic poly(ε-caprolactone) (PCL) mats with antibacterial properties for bone tissue engineering and osteosarcoma prevention. To provide good dispersion of magnetic iron oxide nanoparticles (IONs), they were first grafted with PCL using a novel three-step approach. Then, a series of PCL-based mats containing a fixed amount of ION@PCL particles and an increasing content of ascorbic acid (AA) was prepared by electrospinning. AA is known for increasing osteoblast activity and suppressing osteosarcoma cells. Composites were characterized in terms of morphology, mechanical properties, hydrolytic stability, antibacterial performance, and biocompatibility. AA affected both the fiber diameter and the mechanical properties of the nanocomposites. All produced mats were nontoxic to rat bone marrow-derived mesenchymal cells, however, a composite with 5 wt.% of AA suppressed the initial proliferation of SAOS-2 osteoblast-like cells. Moreover, AA improved antibacterial properties against Staphylococcus aureus and Escherichia coli compared to PCL. Overall, these magnetic composites, reported for the very first time, can be used as scaffolds for both tissue regeneration and osteosarcoma prevention.
Permanent Link: https://hdl.handle.net/11104/0355979