0588500 - ÚMCH 2025 RIV US eng J - Journal Article
Lukáš Petrova, Svetlana - Sincari, Vladimir - Pavlova, Ewa - Pokorný, Václav - Lobaz, Volodymyr - Hrubý, Martin
Microfluidic controlled self-assembly of polylactide (PLA)-based linear and graft copolymers into nanoparticles with diverse morphologies.
ACS Polymers Au. Roč. 4, č. 4 (2024), s. 331-341. E-ISSN 2694-2453
R&D Projects: GA MŠMT(CZ) LM2023053; GA MŠMT(CZ) EH22_008/0004607
Institutional support: RVO:61389013
Keywords : polylactide (PLA)-based copolymers * microfluidic * micelles
OECD category: Polymer science
Impact factor: 4.7, year: 2023
Method of publishing: Open access
Result website:
https://pubs.acs.org/doi/10.1021/acspolymersau.4c00033DOI: https://doi.org/10.1021/acspolymersau.4c00033

This study outlines the microfluidic (MF) controlled self-assembly of polylactide (PLA)-based linear and graft copolymers. The PLA-based copolymers (PLA-Cs) were synthesized through a convenient one-pot/one-step ROP/RAFT technique. Three distinct vinyl monomers─triethylene glycol methacrylate (TEGMA), 2-hydroxypropyl methacrylate (HPMA), and N-(2-hydroxypropyl) methacrylamide (HPMAA) were employed to prepare various copolymers: linear thermoresponsive polylactide-b-poly(triethylene glycol methacrylate) (PLA-b-PTEGMA), graft pseudothermoresponsive poly[N-(2-hydroxypropyl)] methacrylate-g-polylactide (PHPMA-g-PLA), and graft amphiphilic poly[N-(2-hydroxypropyl)] methacrylamide-g-polylactide (PHPMAA-g-PLA). The MF technology was utilized for the controlled self-assembly of these PLA-based BCs in a solution, resulting in a range of nanoparticle (NP) morphologies. The thermoresponsive PLA-b-PTEGMA diblock copolymer formed thermodynamically stable micelles (Ms) through kinetically controlled assemblies. Similarly, employing MF channels led to the self-assembly of PHPMA-g-PLA, yielding polymersomes (PSs) with adjustable sizes under the same solution conditions. Conversely, the PHPMAA-g-PLA copolymer generated worm-like particles (Ws). The analysis of resulting nano-objects involves techniques such as transmission electron microscopy, dynamic light scattering investigations (DLS), and small-angle X-ray scattering (SAXS). More specifically, the thermoresponsive behavior of PLA-b-PTEGMA and PHPMA-g-PLA nano-objects is validated through variable-temperature DLS, TEM, and SAXS methods. Furthermore, the study explored the specific interactions between the formed Ms, PSs, and/or Ws with proteins in human blood plasma, utilizing isothermal titration calorimetry.
Permanent Link: https://hdl.handle.net/11104/0355545