0587891 - FZÚ 2025 RIV CH eng J - Journal Article
Aguedo, J. - Vojs, M. - Vrška, M. - Nemčovič, M. - Pakanová, Z. - Aubrechtová Dragounová, Kateřina - Romanyuk, Olexandr - Kromka, Alexander - Varga, M. - Hatala, M. - Marton, M. - Tkáč, J.
What are the key factors for the detection of peptides using mass spectrometry on boron-doped diamond surfaces?
Nanomaterials. Roč. 14, č. 15 (2024), č. článku 1241. E-ISSN 2079-4991
R&D Projects: GA ČR(CZ) GF23-04322L; GA MŠMT(CZ) EH22_008/0004596
Institutional support: RVO:68378271
Keywords : boron-doped diamond * surface termination * peptides * MALDI * mass spectrometry * nanostructures
OECD category: Condensed matter physics (including formerly solid state physics, supercond.)
Impact factor: 5.3, year: 2022
Method of publishing: Open access

We investigated the use of BDD with different surface morphologies for the enhanced detection of nine different peptides by MALDI-MS. For the first time, we compared three different nanostructured BDD film morphologies with differently terminated surfaces to commercially available Ground Steel plates. All these surfaces were evaluated for their effectiveness in detecting the nine different peptides by MALDI-MS. Our results demonstrated that certain nanostructured BDD surfaces exhibited superior performance for the detection of especially hydrophobic peptides, with a limit of detection of down to 2.3 pM. Further investigation showed that hydrophobic peptides were effectively detected on hydrogen-terminated BDD surfaces. On the other hand, the highly acidic negatively charged peptide adrenocorticotropic hormone fragment 18–39 was effectively identified on oxygen-/fluorine-terminated BDD surfaces. Furthermore, BDD surfaces reduced sodium adduct contamination significantly.
Permanent Link: https://hdl.handle.net/11104/0354963


Research data: Zenodo