Rhodamine B-based Labeling for Oligosaccharide and Glycan Analysis by CE/LIF

0587086 - ÚIACH 2025 CZ eng A - Abstract
Šesták, Jozef - Duša, Filip - Smolková, Denisa - Čmelík, Richard - Guttman, A. - Lavická, Jana
Rhodamine B-based Labeling for Oligosaccharide and Glycan Analysis by CE/LIF.
MSB 2024. Final Program and Abstract Book. Brno: Ústav analytické chemie AV ČR, v. v. i., 2024 - (Lavická, J.; Přikryl, J.; Foret, F.). s. 174-174. ISBN 978-80-908154-1-4.
[MSB 2024. International Symposium on Microscale Separation and Bioanalysis /40./. 19.05.2024-22.05.2024, Brno]
R&D Projects: GA MŠMT(CZ) 9F23002
Institutional support: RVO:68081715
Keywords : fluorescent labeling * rhodamine B * glycans * CE/LIF
OECD category: Analytical chemistry
https://www.msb2024.org/user_uploads/program/Program_and_Abstract_Book_final.pdf

Fluorescent labeling represents a key step in oligosaccharide and glycan analysis by capillary electrophoresis with laser-induced fluorescence detection. In this work, we present a synthesis of the rhodamine B-based fluorescent tag modified by a hydrazide functionality for saccharide derivatization via hydrazone formation chemistry. The labeling conditions were optimized to obtain a high reaction yield. The labeled oligosaccharides and glycans were separated in polybrene-coated capillaries and the separation conditions (BGE compositions, separation, voltage, capillary length, etc.) were optimized. The labeled products were detected with a 532-nm laser and 550-nm longpass or 560/10-nm bandpass optical filters. The optimized labeling and separation conditions were applied for CE/LIF analysis of glycans released from model glycoproteins such as ribonuclease B and immunoglobulin G.
Permanent Link: https://hdl.handle.net/11104/0354381