Analysis of Biothiols in Non-Invasive Sample Matrix with the Use of Gold-Based Nanostructures

0587037 - ÚIACH 2025 CZ eng A - Abstract
Volánek, Jiří - Jonas, Vladimír - Dosedělová, Věra - Kubáň, Petr
Analysis of Biothiols in Non-Invasive Sample Matrix with the Use of Gold-Based Nanostructures.
MSB 2024. Final Program and Abstract Book. Brno: Ústav analytické chemie AV ČR, v. v. i., 2024 - (Lavická, J.; Přikryl, J.; Foret, F.). s. 180-180. ISBN 978-80-908154-1-4.
[MSB 2024. International Symposium on Microscale Separation and Bioanalysis /40./. 19.05.2024-22.05.2024, Brno]
R&D Projects: GA ČR(CZ) GA22-23815S
Institutional support: RVO:68081715
Keywords : exhaled breath condensate (EBC) * gold nanoparticle * biothiols
OECD category: Analytical chemistry
https://www.msb2024.org/user_uploads/program/Program_and_Abstract_Book_final.pdf

In this work, we have focused on the analysis of EBC. A challenging aspect of the EBC analysis is the rather low concentrations of the analytes, which are considerably lower than in plasma or urine samples. We first describe an in-house built device that allows collection of sufficient amount of EBC (several mililiters, at -20 °C). A selective preconcentration by using gold nanoparticles and utilization of gold-thiol interaction, is then employed as a first step. The gold nanoparticles could be used as they are, or, they can be grafted onto a magnetic nanoparticle core, which enables magnetic separation.
Permanent Link: https://hdl.handle.net/11104/0354347