Microfluidic Chip for Cell Lysis: Towards Single-Cell Immunochemistry in Microdroplets

0587017 - ÚIACH 2025 CZ eng A - Abstract
Křivánková, Jana - Weisová, Julie - Hlaváček, Antonín
Microfluidic Chip for Cell Lysis: Towards Single-Cell Immunochemistry in Microdroplets.
MSB 2024. Final Program and Abstract Book. Brno: Ústav analytické chemie AV ČR, v. v. i., 2024 - (Lavická, J.; Přikryl, J.; Foret, F.). s. 155-155. ISBN 978-80-908154-1-4.
[MSB 2024. International Symposium on Microscale Separation and Bioanalysis /40./. 19.05.2024-22.05.2024, Brno]
R&D Projects: GA ČR(CZ) GA21-03156S
Institutional support: RVO:68081715
Keywords : droplet-based microfluidic platform * cell lysis * Microdroplet * immunochemical assays
OECD category: Analytical chemistry
https://www.msb2024.org/user_uploads/program/Program_and_Abstract_Book_final.pdf

Based on previous experimental results, we present a droplet-based microfluidic platform for cell lysis, which integrates a) passive cell encapsulation, b) mixing the microdroplet content, and c) the cell lysis. Microdroplets are produced continuously by pumping fluids (Fluorinert oil and assay buffer containing detergent Triton-X) from external pressure-driven reservoirs. Hela cells in assay buffer stained with Propidium iodine are passively encapsulated into the droplets. Further, the droplet content is mixed and the cells are lysed in the incubation part of the chip. The lysed cells are indicated by red fluorescence. Single-cell
lysates will be mixed with photon-upconversion nanoparticles forming immunochemical complexes, and will be analyzed by massively parallel spectroscopy without separating, washing, and other common steps of
immunochemical assays.
Permanent Link: https://hdl.handle.net/11104/0354337