Liquid Biopsy Testing – Isolation Method for Targeted Nucleic Acid Biomarkers

0586990 - ÚIACH 2025 CZ eng A - Abstract
Hrušková, Helena - Řemínek, Roman - Foret, František
Liquid Biopsy Testing – Isolation Method for Targeted Nucleic Acid Biomarkers.
MSB 2024. Final Program and Abstract Book. Brno: Ústav analytické chemie AV ČR, v. v. i., 2024 - (Lavická, J.; Přikryl, J.; Foret, F.). s. 146-146. ISBN 978-80-908154-1-4.
[MSB 2024. International Symposium on Microscale Separation and Bioanalysis /40./. 19.05.2024-22.05.2024, Brno]
R&D Projects: GA TA ČR(CZ) FW02020209
Institutional support: RVO:68081715
Keywords : PGE-ED * DNA fragments * liquid biopsy testing
OECD category: Analytical chemistry
https://www.msb2024.org/user_uploads/program/Program_and_Abstract_Book_final.pdf

In the presented work, we developed preparative gel electrophoresis combined with an electrodialysis device (PGE-ED). The aim was the isolation of short DNA fragments from the original sample and great emphasis was placed on the reduction of the presence of fragments larger than 1000 base pairs and salts. This purification should result in higher specificity and sensitivity of the subsequent analysis of tumor DNA fragments.
Permanent Link: https://hdl.handle.net/11104/0354317