Analysis of N-linked Glycans by CE/LIF Using Various Glycoproteomic Protocols

0586982 - ÚIACH 2025 CZ eng A - Abstract
Bobálová, Janette - Smolková, Denisa - Strouhalová, Dana - Čmelík, Richard - Lavická, Jana
Analysis of N-linked Glycans by CE/LIF Using Various Glycoproteomic Protocols.
MSB 2024. Final Program and Abstract Book. Brno: Ústav analytické chemie AV ČR, v. v. i., 2024 - (Lavická, J.; Přikryl, J.; Foret, F.). s. 127-127. ISBN 978-80-908154-1-4.
[MSB 2024. International Symposium on Microscale Separation and Bioanalysis /40./. 19.05.2024-22.05.2024, Brno]
R&D Projects: GA ČR(CZ) GA22-00236S
Institutional support: RVO:68081715
Keywords : glycosylation * glycan analysis * glycoproteomic protocol
OECD category: Analytical chemistry
https://www.msb2024.org/user_uploads/program/Program_and_Abstract_Book_final.pdf

Here, we present several modified glycoproteomic protocols that differ, for example, in the purification step, the enzyme used, and the digestion procedure with the aim of efficient and sensitive analysis of N-linked glycans. Ribonuclease B of different concentrations was chosen as a model glycoprotein.The most successful protocol was based on DTT reduction during thermal denaturation. In addition, two reducing agents (DTT and TCEP) were compared, but no significant results were observed.
Permanent Link: https://hdl.handle.net/11104/0354308