Barley MLA3 recognizes the host-specificity effector Pwl2 from Magnaporthe oryzae

0586144 - ÚEB 2025 RIV US eng J - Journal Article
Brabham, H. J. - Gomez De La Cruz, D. - Were, V. - Shimizu, M. - Saitoh, N. - Hernández-Pinzón, I. - Green, P.R. - Lorang, J. - Fujisaki, K. - Sato, K. - Molnár, István - Šimková, Hana - Doležel, Jaroslav - Russell, J. M. - Taylor, J. - Smoker, M. - Gupta, Y. K. - Wolpert, T. - Talbot, N. J. - Terauchi, R. - Moscou, M. J.
Barley MLA3 recognizes the host-specificity effector Pwl2 from Magnaporthe oryzae.
Plant Cell. Roč. 36, č. 2 (2024), s. 447-470. ISSN 1040-4651. E-ISSN 1532-298X
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : powdery-mildew-resistance * leucine-rich repeat * nbs-lrr gene * f-sp hordei * disease-resistance * blast fungus * conferring resistance * convergent evolution * pathogen effector * arabidopsis
OECD category: Biochemistry and molecular biology
Impact factor: 12.0, year: 2022
Method of publishing: Open access
https://doi.org/10.1093/plcell/koad266

Plant nucleotide-binding leucine-rich repeat (NLRs) immune receptors directly or indirectly recognize pathogen-secreted effector molecules to initiate plant defense. Recognition of multiple pathogens by a single NLR is rare and usually occurs via monitoring for changes to host proteins, few characterized NLRs have been shown to recognize multiple effectors. The barley (Hordeum vulgare) NLR gene Mildew locus a (Mla) has undergone functional diversification, and the proteins encoded by different Mla alleles recognize host-adapted isolates of barley powdery mildew (Blumeria graminis f. sp. hordei [Bgh]). Here, we show that Mla3 also confers resistance to the rice blast fungus Magnaporthe oryzae in a dosage-dependent manner. Using a forward genetic screen, we discovered that the recognized effector from M. oryzae is Pathogenicity toward Weeping Lovegrass 2 (Pwl2), a host range determinant factor that prevents M. oryzae from infecting weeping lovegrass (Eragrostis curvula). Mla3 has therefore convergently evolved the capacity to recognize effectors from diverse pathogens.
Permanent Link: https://hdl.handle.net/11104/0353729