Impact of media supplements FGF2, LIF and IGF1 on the genome activity of porcine embryos produced in vitro

0585295 - ÚŽFG 2025 RIV US eng J - Journal Article
Rosenbaum Bartková, Alexandra - Němcová, Lucie - Strejček, F. - Gad, A. - Kinterová, Veronika - Morovič, M. - Benc, M. - Procházka, Radek - Laurinčik, J.
Impact of media supplements FGF2, LIF and IGF1 on the genome activity of porcine embryos produced in vitro.
Scientific Reports. Roč. 14, č. 1 (2024), č. článku 7081. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA MŠMT EF15_003/0000460; GA ČR(CZ) GA23-05108S
Institutional support: RVO:67985904
Keywords : gene-expression * growth-factor * oocytes * maturation * fertilization
OECD category: Developmental biology
Impact factor: 4.6, year: 2022
Method of publishing: Open access
https://www.nature.com/articles/s41598-024-57865-7

In this article, we focused on the impact of precisely chemically modified FLI maturation medium enriched with fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), insulin-like growth factor 1 (IGF1), and polyvinyl alcohol (PVA) and its potential to improve the efficiency of in vitro production of porcine embryos. We hypothesized that enhancing the composition of the maturation medium could result in an elevated production of embryos in vitro and can affect EGA. FLI medium resulted in a significantly higher rate of oocyte blastocyst maturation and formation compared to the control DMEM medium. In addition, immunocytochemical labelling confirmed the detection of UBF in 4-cell FLI parthenogenic embryos, suggesting similarities with natural embryo development. Through RNAseq analysis, upregulated genes present in 4-cell FLI embryos were found to play key roles in important biological processes such as cell proliferation, cell differentiation, and transcriptional regulation. Based on our findings, we demonstrated the positive influence of FLI medium in the evaluation of in vitro embryo production, EGA detection, transcriptomic and proteomic profile, which was confirmed by the positive activation of the embryonal genome in the 4-cell stage of parthenogenetically activated embryos.
Permanent Link: https://hdl.handle.net/11104/0353024