A New Method for the Production of High-Concentration Collagen Bioinks with Semiautonomic Preparation

0584953 - ÚSMH 2025 RIV CH eng J - Journal Article
Matějková, J. - Kaňoková, D. - Šupová, Monika - Matějka, R.
A New Method for the Production of High-Concentration Collagen Bioinks with Semiautonomic Preparation.
Gels. Roč. 10, č. 1 (2024), č. článku 66. E-ISSN 2310-2861
R&D Projects: GA MZd(CZ) NV19-02-00068
Institutional support: RVO:67985891
Keywords : bioprinting * bioink * collagen hydrogels * biofabrication * pH * neutralization * automation * stromal cells
OECD category: Polymer science
Impact factor: 4.6, year: 2022
Method of publishing: Open access
https://doi.org/10.3390/gels10010066

It is believed that 3D bioprinting will greatly help the field of tissue engineering and regenerative medicine, as live patient cells are incorporated into the material, which directly creates a 3D structure. Thus, this method has potential in many types of human body tissues. Collagen provides an advantage, as it is the most common extracellular matrix present in all kinds of tissues and is, therefore, very natural for cells and the organism. Hydrogels with highly concentrated collagen make it possible to create 3D structures without additional additives to crosslink the polymer, which could negatively affect cell proliferation and viability. This study established a new method for preparing highly concentrated collagen bioinks, which does not negatively affect cell proliferation and viability. The method is based on two successive neutralizations of the prepared hydrogel using the bicarbonate buffering mechanisms of the 2× enhanced culture medium and pH adjustment by adding NaOH. Collagen hydrogel was used in concentrations of 20 and 30 mg/mL dissolved in acetic acid with a concentration of 0.05 and 0.1 wt.%. The bioink preparation process is automated, including colorimetric pH detection and adjustment. The new method was validated using bioprinting and subsequent cultivation of collagen hydrogels with incorporated stromal cells. After 96 h of cultivation, cell proliferation and viability were not statistically significantly reduced.
Permanent Link: https://hdl.handle.net/11104/0352743