Isolation and characterization of multiple-stress tolerant bacteria from radon springs

0584829 - MBÚ 2025 RIV US eng J - Journal Article
Timkina, E. - Kulišová, M. - Palyzová, Andrea - Marešová, Helena - Maťátková, O. - Řezanka, Tomáš - Jarošová Kolouchová, I.
Isolation and characterization of multiple-stress tolerant bacteria from radon springs.
PLoS ONE. Roč. 19, č. 3 (2024), č. článku e0299532. ISSN 1932-6203. E-ISSN 1932-6203
Institutional support: RVO:61388971
Keywords : radiotolerans sp nov. * radiation-resistant * genus rhodococcus * acid-composition * fatty-acids * diversity * waters * geomicrobiology * microorganisms * extremophiles
OECD category: Microbiology
Impact factor: 3.7, year: 2022
Method of publishing: Open access
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0299532

Radon springs, characterized by their high concentrations of radon gas (Rn222), are extreme environments with unique physicochemical conditions distinct from conventional aquatic ecosystems. Our research aimed to investigate microbial life in radon springs, focusing on isolating extremophilic bacteria and assessing their resistance to adverse conditions. Our study revealed the prevalence of Actinomycetia species in the radon spring environment. We conducted various tests to evaluate the resistance of these isolates to oxidative stress, irradiation, desiccation, and metal ion content. These extremophilic bacteria showed overall higher resistance to these stresses compared to control strains. Lipidomic analysis was also employed to provide insights into the adaptive mechanisms of these bacteria which were found mainly in the correlations among individual clusters and changes in content of fatty acids (FA) as well as differences between content and type of FAs of environmental isolates and type strains.
Permanent Link: https://hdl.handle.net/11104/0353076