Methodology for co-cryostorage of virus isolates in their host plants and relevant data

0584637 - BC 2024 RIV CZ eng L3 - Certified Methodologys
Bilavčík, A. - Bjerkestrand, R. - Bjelland, B. - Blystad, D.-R. - Bobrova, O. - Fránová, Jana - Faltus, M. - Hamborg, Z. - Hammond Hammond, S. D. - Koloniuk, Igor - Zámečník, J.
Methodology for co-cryostorage of virus isolates in their host plants and relevant data.
Internal code: 7/23 ; 2023
Technical parameters: Osvědčení o uznání metodiky č.j. MZE-73887/2023-13113 vydalo Ministerstvo zemědělství, Odbor environmentálního a ekologického zemědělství dne 22.12.2023. Smlouva o uplatnění metodiky č. 18/2023 byla uzavřena dne 20.12.2023 mezi Výzkumným ústavem rostlinné výroby, v. v. i., Drnovská 507/73, 161 06 Praha 6, IČ: 00027006, DIČ: CZ00027006 (poskytovatel) a Ministerstvo zemědělství, Odbor environmentální a ekologického zemědělství, Těšnov 65/17, 110 00 Praha 1
Economic parameters: Cryopreservation is economically beneficial, saving storage space and preserving material for biotech applications. Co-storing virus isolates with data reduces the risk of loss, a key advantage for users.
Certifying body: Ministerstvo zemědělství, Odbor environmentální a ekologického zemědělství, Těšnov 65/17, 110 00 Praha 1, ČR. Certificate issue date: 22.12.2023
R&D Projects: GA TA ČR(CZ) TO01000295
Institutional support: RVO:60077344
Keywords : genetic resources * virus * Rubus idaeus L.
OECD category: Agronomy, plant breeding and plant protection
https://www.vurv.cz/wp-content/uploads/2024/01/V13_Methodology-for-co-cryostorage-of-virus-isolates-in-their-host-plants-and-relevant-data.pdf

Currently, the nursery production of plant material emphasizes the production of healthy, virus-free material. For this reason, it is necessary to have reference plant materials in which the virus is present. Some types of viruses are difficult to maintain outside the plant host. Because of the potential transmission to outdoor plantings, it is necessary to keep infected plants in special cultivation areas, and net-houses, which brings considerable financial and labour costs. Therefore, a methodology was developed in which the virus status in plant material is analysed, the infected material can be stored using cryopreservation in liquid nitrogen and then regenerated in vitro, and relevant data in GeneEver cryobox can be stored.
Permanent Link: https://hdl.handle.net/11104/0352523