Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin

0584554 - ÚIACH 2025 RIV NL eng J - Journal Article
Hrušková, H. - Olsen, C. - Řemínek, Roman - Wang, C. - Aizenshtadt, A. - Krauss, S. - Scholz, H. - Roberg-Larsen, H. - Foret, František - Wilson, S.R.
Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin.
Journal of Chromatography A. Roč. 1717, FEB (2024), č. článku 464669. ISSN 0021-9673. E-ISSN 1873-3778
Institutional support: RVO:68081715
Keywords : proteins * liquid chromatography-mass spectrometry * preparative gel electrophoresis
OECD category: Analytical chemistry
Impact factor: 4.1, year: 2022
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0021967324000426?via%3Dihub

Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LCMS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LCMS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LCMS analysis of human insulin.
Permanent Link: https://hdl.handle.net/11104/0352463