Soft hydrogels with double porosity modified with RGDS for tissue engineering

0584552 - ÚMCH 2025 RIV US eng J - Journal Article
Podhorská, Bohumila - Chylíková Krumbholcová, Eva - Dvořáková, Jana - Šlouf, Miroslav - Kobera, Libor - Pop-Georgievski, Ognen - Frejková, Markéta - Proks, Vladimír - Janoušková, Olga - Filipová, Marcela - Chytil, Petr
Soft hydrogels with double porosity modified with RGDS for tissue engineering.
Macromolecular Bioscience. Roč. 24, č. 3 (2024), č. článku 2300266. ISSN 1616-5187. E-ISSN 1616-5195
R&D Projects: GA ČR(CZ) GA21-06524S
Institutional support: RVO:61389013
Keywords : double porosity hydrogels * hydrogels * mesenchymal stem cells
OECD category: Polymer science
Impact factor: 4.6, year: 2022
Method of publishing: Open access
https://onlinelibrary.wiley.com/doi/10.1002/mabi.202300266

This study develops and characterizes novel biodegradable soft hydrogels with dual porosity based on N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers cross-linked by hydrolytically degradable linkers. The structure and properties of the hydrogels are designed as scaffolds for tissue engineering and they are tested in vitro with model mesenchymal stem cells (rMSCs). Detailed morphological characterization confirms dual porosity suitable for cell growth and nutrient transport. The dual porosity of hydrogels slightly improves rMSCs proliferation compared to the hydrogel with uniform pores. In addition, the laminin coating supports the adhesion of rMSCs to the hydrogel surface. However, hydrogels modified by heptapeptide RGDSGGY significantly stimulate cell adhesion and growth. Moreover, the RGDS-modified hydrogels also affect the topology of proliferating rMSCs, ranging from single-cell to multicellular clusters. The 3D reconstruction of the hydrogels with cells obtained by laser scanning confocal microscopy (LSCM) confirms cell penetration into the inner structure of the hydrogel and its corresponding microstructure. The prepared biodegradable oligopeptide-modified hydrogels with dual porosity are suitable candidates for further in vivo evaluation in soft tissue regeneration.
Permanent Link: https://hdl.handle.net/11104/0353390