Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato

0584344 - BC 2024 RIV CH eng J - Journal Article
Petrzik, Karel - Vacek, J. - Kmoch, M. - Binderová, D. - Brázdová, Sára - Lenz, Ondřej - Ševčík, R.
Field Use of Protective Bacteriophages against Pectinolytic Bacteria of Potato.
Microorganisms. Roč. 11, č. 3 (2023), č. článku 620. E-ISSN 2076-2607
R&D Projects: GA MZe(CZ) QK1910028
Institutional support: RVO:60077344
Keywords : Enterobacteriaceae * Solanum tuberosum * Limestonevirus * biological control
OECD category: Virology
Impact factor: 4.5, year: 2022
Method of publishing: Open access
https://www.mdpi.com/2076-2607/11/3/620

The pectinolytic Dickeya solani bacterium is an important pathogen found in potatoes. We conducted laboratory and field experiments mimicking severe and mild Dickeya spp. infection and investigated the application of a mixture of two lytic bacteriophages before and after bacterial infection to protect the plants. Application of the phage solution to tuber disks and wounded tubers did not completely eliminate the infection but reduced the development of soft rot symptoms by 59.5-91.4%, depending on the phage concentration. In the field trial, plants treated with bacteriophages after severe Dickeya infection had 5-33% greater leaf cover and 4-16% greater tuber yield compared to untreated plants. When simulating a mild infection, leaf cover was 11-42% greater, and tuber yield was 25-31% greater compared to untreated plants. We conclude that the phage mixture has the potential to protect potatoes ecologically from D. solani.
Permanent Link: https://hdl.handle.net/11104/0352330