Elimination of Solanum nigrum ilarvirus 1 and Apple Hammerhead Viroid from Apple Cultivars Using Antivirals Ribavirin, Rimantadine, and Zidovudine

0584331 - BC 2024 RIV CH eng J - Journal Article
Sedlák, J. - Přibylová, Jaroslava - Koloniuk, Igor - Špak, Josef - Lenz, Ondřej - Semerák, M.
Elimination of Solanum nigrum ilarvirus 1 and Apple Hammerhead Viroid from Apple Cultivars Using Antivirals Ribavirin, Rimantadine, and Zidovudine.
Viruses. Roč. 15, č. 8 (2023), č. článku 1684. E-ISSN 1999-4915
R&D Projects: GA MZe(CZ) QK1910065
Institutional support: RVO:60077344
Keywords : apple * virus * viroid * hts * chemotherapy
OECD category: Virology
Impact factor: 4.7, year: 2022
Method of publishing: Open access
https://www.mdpi.com/1999-4915/15/8/1684

Apple hammerhead viroid (AHVd) was detected in the apple cultivar Sampion and in mixed infection with Solanum nigrum ilarvirus 1 (SnIV-1) in the cultivars Selena and Jonagored Supra, using a high-throughput sequencing method. Experiments were conducted to eliminate both pathogens in apples using meristem tip cultures in combination with the antivirotics ribavirin, rimantadine, and zidovudine. Elimination of both pathogens was verified by repeated RT-PCR and qRT-PCR assays after 7-11 months. Elimination of SnIV-1 from all cultivars was successful with each of the three antivirotics at concentrations of 20, 40, and 80 mg L-1. Elimination of AHVd was also achieved, although less effectively and only with ribavirin in the concentration range of 20-160 mg L-1.
Permanent Link: https://hdl.handle.net/11104/0352335