Plant Synthetic Promoters: Advancement and Prospective

0584252 - BC 2024 RIV CH eng J - Journal Article
Khan, Ahamed - Nasim, N. - Pudhuvai, B. - Koul, B. - Upadhyay, S. K. - Sethi, L. - Dey, N.
Plant Synthetic Promoters: Advancement and Prospective.
Agriculture-Basel. Roč. 13, č. 2 (2023), č. článku 298. E-ISSN 2077-0472
Institutional support: RVO:60077344
Keywords : synthetic promoter * crispr * Cis-engineering * transcription factors * gene expression
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 3.6, year: 2022
Method of publishing: Open access
https://www.mdpi.com/2077-0472/13/2/298

Native/endogenous promoters have several fundamental limitations in terms of their size, Cis-elements distribution/patterning, and mode of induction, which is ultimately reflected in their insufficient transcriptional activity. Several customized synthetic promoters were designed and tested in plants during the past decade to circumvent such constraints. Such synthetic promoters have a built-in capacity to drive the expression of the foreign genes at their maximum amplitude in plant orthologous systems. The basic structure and function of the promoter have been discussed in this review, with emphasis on the role of the Cis-element in regulating gene expression. In addition to this, the necessity of synthetic promoters in the arena of plant biology has been highlighted. This review also provides explicit information on the two major approaches for developing plant-based synthetic promoters: the conventional approach (by utilizing the basic knowledge of promoter structure and Cis-trans interaction) and the advancement in gene editing technology. The success of plant genetic manipulation relies on the promoter efficiency and the expression level of the transgene. Therefore, advancements in the field of synthetic promoters has enormous potential in genetic engineering-mediated crop improvement.
Permanent Link: https://hdl.handle.net/11104/0352316