Holocentromeres can consist of merely a few megabase-sized satellite arrays

0584244 - BC 2024 RIV US eng J - Journal Article
Kuo, Y. T. - Câmara, A. S. - Schubert, V. - Neumann, Pavel - Macas, Jiří - Melzer, M. - Chen, J. - Fuchs, J. - Abel, S. - Klocke, E. - Huettel, B. - Himmelbach, A. - Demidov, D. - Dunemann, F. - Mascher, M. - Ishii, T. - Marques, A. - Houben, A.
Holocentromeres can consist of merely a few megabase-sized satellite arrays.
Nature Communications. Roč. 14, č. 1 (2023), č. článku 3502. E-ISSN 2041-1723
R&D Projects: GA ČR(CZ) GA20-25440S
Institutional support: RVO:60077344
Keywords : nuclear-dna content * histone h3 * chionographis liliaceae * luzula-elegans * centromere * chromosomes * arabidopsis * methylation * euchromatin * generation
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 16.6, year: 2022
Method of publishing: Open access
https://www.nature.com/articles/s41467-023-38922-7

The centromere is the chromosome region where microtubules attach during cell division. In contrast to monocentric chromosomes with one centromere, holocentric species usually distribute hundreds of centromere units along the entire chromatid. We assembled the chromosome-scale reference genome and analyzed the holocentromere and (epi)genome organization of the lilioid Chionographis japonica. Remarkably, each of its holocentric chromatids consists of only 7 to 11 evenly spacedmegabase-sized centromere-specific histone H3-positive units. These units contain satellite arrays of 23 and 28 bp-long monomers capable of forming palindromic structures. Like monocentric species, C. japonica forms clustered centromeres in chromocenters at interphase. In addition, the large-scale eu- and heterochromatin arrangement differs between C. japonica and other known holocentric species. Finally, using polymer simulations, we model the formation of prometaphase line-like holocentromeres from interphase centromere clusters. Our findings broaden the knowledge about centromere diversity, showing that holocentricity is not restricted to species with numerous and small centromere units.
Permanent Link: https://hdl.handle.net/11104/0352321